Chile coach Reinaldo Rueda confirmed Alexis Sanchez had suffered an ankle injury.
Chile overcame Ecuador at the Copa America to move into the quarter-finals from Group C.
Ecuador were thrashed by Uruguay last time out and must now look to bounce back against two-time defending Copa America champions Chile.
A hamstring injury to Matias Vecino has provided Uruguay with an early setback at the Copa America.
Luis Suarez and Edinson Cavani suffered significant injuries earlier this year, but they starred in Uruguay's Copa America opener.
Edinson Cavani revelled in Sunday's result as Uruguay – Copa America's most successful team – eye their first title since 2011.
Luis Suarez and Edinson Cavani were on the scoresheet in Uruguay's comfortable win over Ecuador at the Copa America.
Fifteen-time champions Uruguay begin their latest bid for Copa America glory with a showdown against out-of-form Ecuador in Belo Horizonte.
Brazil get the 2019 Copa America started on Friday and the experts have identified the players to watch for each of the 12 nations.
Neymar will be missing, but the show must go on for Copa America hosts Brazil, who will be hoping to banish memories of the 2014 World Cup.