Italy boss Roberto Mancini handed first call-ups to Atalanta captain Rafael Toloi and Spezia midfielder Matteo Ricci as he selected a 38-man squad for the three upcoming World Cup qualifiers.
Brazilian-born Toloi has been residing in Italy since leaving Sao Paulo to join Atalanta in 2015 and has received the citizenship that makes him eligible to represent the Azzurri.
The 30-year-old has been summoned at the first opportunity by head coach Mancini, who has observed the centre-back as a prominent member of the Atalanta team that has caught the eye in Serie A and the Champions League.
Ricci, 26, joined Spezia from Roma in July 2018 and helped the team win promotion to Serie A last season.
He has yet to score in the top flight for Spezia this term but has had two assists and created 16 chances, while winning possession 139 times, a team-high mark.
Italy face Northern Ireland in Parma in their Group C qualifying opener next Thursday, then tackle Bulgaria in Sofia on March 28 before playing Lithuania three days later in Vilnius.
The Inter trio of Alessandro Bastoni, Nicolo Barella and Stefano Sensi will only be involved if they have the all-clear from health authorities, given they are currently subject to COVID-19 restrictions after positive tests at the club.
Inter's Stefan de Vrij and Matias Vecino tested positive for coronavirus, and the Nerazzurri said on Thursday all players would be prevented from joining up with their��international teams, although that may change if they return negative test results on Monday.
The Italian Football Federation (FIGC) said Ricci must also be similarly cleared to join up.
Italy are unbeaten in 22 games and have qualified for both Euro 2020, which takes place in June and July, and the Nations League Finals in October.
Italy squad: Alessio Cragno (Cagliari), Gianluigi Donnarumma (Milan), Alex Meret (Napoli), Salvatore Sirigu (Torino); Francesco Acerbi (Lazio), Alessandro Bastoni (Inter), Cristiano Biraghi (Fiorentina), Leonardo Bonucci (Juventus), Giorgio Chiellini (Juventus), Giovanni Di Lorenzo (Napoli), Emerson Palmieri (Chelsea), Gian Marco Ferrari (Sassuolo), Alessandro Florenzi (Paris Saint-Germain), Manuel Lazzari (Lazio), Gianluca Mancini (Roma), Leonardo Spinazzola (Roma), Rafael Toloi (Atalanta); Nicolo Barella (Inter), Gaetano Castrovilli (Fiorentina), Bryan Cristante (Roma), Manuel Locatelli (Sassuolo), Rolando Mandragora (Torino), Lorenzo Pellegrini (Roma), Matteo Pessina (Atalanta), Matteo Ricci (Spezia), Stefano Sensi (Inter), Roberto Soriano (Bologna), Marco Verratti (Paris Saint-Germain); Andrea Belotti (Torino), Domenico Berardi (Sassuolo), Federico Bernardeschi (Juventus), Francesco Caputo (Sassuolo), Federico Chiesa (Juventus), Stephan El Shaarawy (Roma), Vincenzo Grifo (Friburgo), Ciro Immobile (Lazio), Lorenzo Insigne (Napoli), Moise Kean (Paris Saint-Germain).