A group of Australian Open players and staff will quarantine for two weeks after two people on their flight to Melbourne tested positive for coronavirus.

About 1,200 players and staff are arriving in Melbourne ahead of the delayed Australian Open starting on February 8.

But one flight, from Los Angeles which landed on Friday, has led to two positive COVID-19 tests, a letter shared by players Pablo Cuevas and Santiago Gonzalez said.

"Unfortunately, we have been informed by the health authorities that two people on your flight QR7493 from LAX that arrived at 5.15am on Friday 15 January have returned positive COVID-19 PCR tests on arrival in Melbourne," part of the letter read.

"The chief health officer has reviewed the flight and has determined that everyone on board needs to isolate and will be confined to their rooms for the 14 day quarantine period."

The two positive tests were a crew member and a participant who is not a player.

Players were due to be allowed to train for five hours per day, but those on that flight will now quarantine for two weeks.

"All remaining 66 passengers on the flight have been determined to be close contacts," the Department of Health and Human Services (DHHS) said.

"Any players and support people will not be able to leave quarantine to attend training."

Victoria on Saturday recorded its 10th consecutive day with no locally acquired cases of coronavirus.