For the first time in Wimbledon history not one of the top-10 seeds in the women's draw made it into the last eight.
A day on from Petra Kvitova's surprise Wimbledon departure, Caroline Wozniacki joined the pre-tournament favourite in making an early exit.
Caroline Wozniacki was bugged by insects, court conditions and the form of Ekaterina Makarova as she crashed out of Wimbledon.
Seven-time Wimbledon champion Serena Williams picked up where she left off in 2016 with victory over Arantxa Rus on Monday.
World number three Garbine Muguruza will not allow herself to be consumed by the pressure of defending her Wimbledon title.
Aryna Sabalenka looked to be in with a real chance of a first WTA Tour title, but she let Caroline Wozniacki in at Eastbourne.
Caroline Wozniacki will be strongly fancied to start Wimbledon on the back of a second title in Eastbourne after beating Angelique Kerber.
Agnieszka Radwanska expressed her delight at reaching the last four of the Nature Valley International - despite not packing enough clothes!
Johanna Konta previously had the upper hand over Caroline Wozniacki, but the world number two came out on top at Eastbourne.
After meeting in the final at Eastbourne last year, Karolina Pliskova and Caroline Wozniacki are on course for another clash after wins.