Marc Marquez's Honda stand-in Stefan Bradl will not race at Misano this weekend because of an arm problem.
Marc Marquez is set to miss the bulk of the MotoGP season after Repsol Honda announced he could be out for another three months.
Maverick Vinales returned to form in qualifying while Fabio Quartararo is on the front row for the Austrian Grand Prix.
After struggling on Friday, Maverick Vinales found form to qualify first for the Austrian Grand Prix.
If Fabio Quartararo is to capitalise on Marc Marquez's injury and pull clear in MotoGP, improvement is required in Austria this week.
It was a historic day for Brad Binder and his KTM team in Brno in another MotoGP thriller.
It was a day of firsts for KTM and Brad Binder, who had not yet quite comprehended his achievement at winning in MotoGP.
Johann Zarco claimed pole position ahead of Fabio Quartararo, who will be aiming for a hat-trick in Sunday's race.
There is a Frenchman on pole for the Czech Republic Grand Prix but it is Johann Zarco rather than Fabio Quartararo at the front of the pack.
The setback in Marc Marquez's recovery came about by accident after an innocuous incident at home.