Sam Underhill is the latest player to withdraw from England's Six Nations squad due to a hip injury.
Jack Willis has been called up as a replacement for back-row Underhill, who is another big loss for the defending champions.
Underhill played a big part in England's Six Nations and Autumn Nations Cup triumphs last year but sustained an injury blow before he was due to link up with the squad at St. George's Park on Wednesday.
Willis made his Test debut against Georgia last November and will be hoping to make his Six Nations bow in the coming weeks.
Underhill's withdrawal comes a day after Joe Launchbury and Joe Marler were ruled out due to a fibula stress fracture and personal reasons respectively.
England head coach Eddie Jones' preparations had already been disrupted last week when he was forced to go into self-isolation after his assistant Matt Proudfoot tested positive for coronavirus.
The Red Rose start the defence of their title against Scotland at Twickenham a week on Saturday.