Josh Papalii and Joe Tapine have been cleared to face North Queensland Cowboys on Saturday after the Canberra Raiders pair, who refused flu vaccines, were given medical exemptions.
Queensland chief health officer Jeannette Young said in May that NRL players who chose to skip the vaccination drive would not be allowed to play in the state.
The NRL had called on all players in the competition to be vaccinated, in line with efforts to bolster its biosecurity measures during the coronavirus crisis.
Papalii said in May that the decision not to be vaccinated was "a personal thing for me and my family".
However, both he and Tapine have received the go-ahead to take part in this weekend's game.
The Raiders said in a statement: "Canberra Raiders Josh Papalii and Joe Tapine have been cleared by the Queensland Government to travel and play matches in Queensland.
"They have both received exemptions on medical grounds.
"The club will be making no further comment on this matter due to the privacy regarding the medical exemptions."