Frenkie de Jong and Jordi Alba were granted a rest as Ronald Koeman called Alex Collado up for Barcelona's Copa del Rey clash with Cornella on Thursday.
Koeman gave a break to De Jong (2,166) and Alba (1,927), who rank first and third respectively for minutes played in all competitions at Barca this season.
Lionel Messi (1,939) separates them but misses the trip to the Segunda B side as he serves the first game of a two-match ban following his sending off in the Supercopa de Espana final defeat to Athletic Bilbao on Sunday. The suspension could yet be reduced to one game as Barca lodged an appeal.
Koeman has taken the chance to include 21-year-old Barcelona B midfielder Collado in a matchday squad for the first time since taking over in August.
Collado made his Barca debut in May 2019 but has only managed one further first-team appearance since then.
Fellow Barca B players Ilaix Moriba, Arnau Tenas and Konrad de la Fuente have also been named in the 20-man group.
Koeman still has plenty of experienced players available to him and will hope to avoid a shock exit like Atletico Madrid and Real Madrid have suffered this season.
Cornella stunned Atletico in the second round and will be keen for another scalp, while Madrid were on the receiving end of a 2-1 loss to 10-man Alcoyano after extra time on Wednesday.