Juventus wide player Juan Cuadrado has tested positive for coronavirus and will miss the clash with Serie A leaders Milan on Wednesday.
The Colombia international is asymptomatic but has begun a period of self-isolation, the Italian champions confirmed.
The news comes just a day after left-back Alex Sandro was ruled out after returning a positive test for the virus.
It means head coach Andrea Pirlo will be without two of his first-choice full-backs for the game at San Siro against Stefano Pioli's side, who are the only unbeaten team in Europe's top five leagues in the 2020-21 season.
The club said in a statement: "Juventus Football Club announces that, during the checks provided for by the protocol in force, Juan Cuadrado has emerged positive with COVID-19. The player has already been placed in solitary confinement and is asymptomatic."
Cuadrado, 32, has made 13 appearances in Serie A this term, scoring once.
Juve head into the Milan contest with a 10-point deficit to the league leaders, albeit with a game in hand.
While they have lost only once in 14 matches, Juve have won just seven times, leaving them in fifth place with 27 points.
They are hoping to avoid losing back-to-back league games against the Rossoneri for the first time since 2010, having been beaten 4-2 in the previous meeting last July.
The last time they faced Milan when behind them in the table was in November 2015, when a Paulo Dybala goal secured a 1-0 victory.