The pots are set for the last 16, with the draw scheduled to take place in Paraguay on Monday.
River Plate progressed to the Copa Libertadores knockout stage after a win at Palestino.
Boca Juniors scored four goals against Jorge Wilstermann on Wednesday, after Cruzeiro's Fred celebrated a first-half hat-trick.
Nicolas De La Cruz's spectacular free-kick helped River Plate earn a draw at Internacional in the Copa Libertadores.
Lucas Viatri led Penarol to a comprehensive win over San Jose in the Copa Libertadores.
Last season's runners-up Boca Juniors were held by Bolivian hosts Jorge Wilstermann in Group G on Tuesday.
We assess the leading candidates for glory and the potential stars ahead of the start of the 2019 Copa Libertadores.
Wilstermann coach Alvaro Pena blasted Universitario after a wages issues saw them play youth players who forfeited the game when losing 7-0.
River Plate and Lanus reached the Copa Libertadores semi-finals after overturning first-leg deficits.
Edward Zenteno, Gilbert Alvarez and Cristhian Machado led Wilstermann to a first-leg win over River Plate.