The top two in the Top 14 both triumphed on Saturday, with Bordeaux-Begles and Lyon improving to three wins from three this season.
Toulouse won the Top 14 last season but they have been beaten in each of their first two matches at the start of the 2019-20 campaign.
The second round of Top 14 fixtures saw hard-fought wins for Clermont Auvergne, La Rochelle and Montpellier, among others, on Saturday.
Clermont Auvergne are up and running in the Top 14 as Peter Betham inspired a 28-10 win over La Rochelle on Sunday.
There were shock wins for Bayonne and Bordeaux-Begles as the Top 14 returned for the 2019-20 season on Saturday.
After Toulouse beat Clermont Auvergne to win the Top 14 title for the 20th time, Ugo Mola paid tribute to his players.
Toulouse won the Top 14 final for the first time in seven years thanks to Yoann Huget's double against Clermont Auvergne.
Ronan O'Gara will take the next step in his coaching career with Top 14 side La Rochelle after the Irishman opted to leave the Crusaders.
The teams who finished first and second in the Top 14 regular season will contest the final after Clermont Auvergne got the better of Lyon.
Toulouse booked their place in a Top 14 final for the first time since 2012 after they overcame La Rochelle in Bordeaux.