Venus Williams upset Kiki Bertens as five seeds fell at the Western & Southern Open.
Maria Sharapova – a five-time grand slam champion – was eliminated following a two hour, 41-minute thriller in Toronto on Monday.
Rainer Schuttler's spell working with Angelique Kerber is over after the three-time grand slam champion confirmed their split on Twitter.
Maria Sharapova, Venus Williams and Angelique Kerber have achieved a feat that Simona Halep has in her sights at SW19.
Serena Williams still has plans for the weekend at Wimbledon after winning on Thursday, but Angelique Kerber is heading home early.
Lauren Davis was hampered by three apparent injuries against Angelique Kerber but still dumped the defending Wimbledon champion out.
Angelique Kerber began her title defence by surviving a battle of wits at Wimbledon as she beat fellow German Tatjana Maria on Centre Court.
Serena Williams potentially lies in Angelique Kerber's way at Wimbledon, but the defending champion is not giving it too much thought.
World number three Karolina Pliskova has been installed as joint favourite to win Wimbledon after landing her third title of the year.
Angelique Kerber simply had no riposte to the brilliance of Karolina Pliskova, who heads into Wimbledon as a champion in Eastbourne.