Christian Pulisic is out of Borussia Dortmund's Bundesliga game at home to Wolfsburg, head coach Lucien Favre has confirmed.
Pulisic sustained a quad injury on international duty with United States and is not available for Saturday's match, with Lukasz Piszczek and Maximilian Philipp also absent.
Dortmund are level on points with Bayern Munich at the top of the Bundesliga table, although the defending champions lead on goal difference ahead of the April 6 meeting between the sides at the Allianz Arena.
Favre, however, will have to do without Pulisic, who will join Chelsea ahead of the 2019-20 season, for the Wolfsburg clash.
"All of the players returned fit except for Christian Pulisic," Favre told reporters. "Unfortunately, he won't play on Saturday for sure.
"They are okay. They all took part in training. [Mario] Gotze, [Paco] Alcacer and [Axel] Witsel trained the whole week.
"Everything is fine. Still, Lukasz Piszczek and Maximilian Philipp are not ready for the match."
Gotze has been one of Dortmund's key men in their title charge and sporting director Michael Zorc confirmed the club are keen to offer the playmaker a new deal.
"We all realised that Mario Gotze is improving throughout the season and I hope he is not done yet. I hope he will keep improving," said Zorc.
"Regarding contract talks, we don't want to announce them officially but want to talk with the player personally."