Twickenham will host two NFL fixtures in 2017 as the league takes four matches to London for the first time.
Wembley has hosted 16 games since NFL made its debut at the home of English football in 2007, with Twickenham branching out for the first time to host the Los Angeles Rams' home clash with the New York Giants in October.
And the Rams will once again call the national rugby stadium home when they take on the Arizona Cardinals, with the Cleveland Browns welcoming the Minnesota Vikings to the same venue.
Wembley, meanwhile, hosts the Miami Dolphins against the New Orleans Saints, as well as the Jacksonville Jaguars' clash with the Baltimore Ravens.
The Wembley fixtures will take place in weeks three and four, with Twickenham taking centre stage in weeks seven and eight.
For the Cardinals, the Ravens and the Browns, the fixtures will mark their first regular-season trips to London.
"We are looking forward to a London season that will feature many firsts, including three new teams," said Alistair Kirkwood, managing director of NFL UK.
"Our fans have the chance to watch star players such as Baltimore's Super Bowl-winning quarter-back, Joe Flacco, and one of the greatest-ever wide receivers in Larry Fitzgerald of the Cardinals.
"Having one of the most famous fan groups in the NFL, Cleveland's Dawg Pound, sharing Twickenham with our UK fans is going to be a lot of fun, and we are excited to be welcoming home a Londoner, Dolphins running back Jay Ajayi, when his team plays at Wembley."
NFL London fixtures:
— Week 3 or 4 (Sept. 24 or Oct. 1): Baltimore Ravens v Jacksonville Jaguars at Wembley
— Week 3 or 4 (Sept. 24 or Oct. 1): New Orleans Saints v Miami Dolphins at Wembley
— Week 7 or 8 (Oct. 22 or 29): Minnesota Vikings v Cleveland Browns at Twickenham
— Week 7 or 8 (Oct. 22 or 29): Arizona Cardinals v Los Angeles Rams at Twickenham